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Fig. 4 | BMC Genomics

Fig. 4

From: Split Pool Ligation-based Single-cell Transcriptome sequencing (SPLiT-seq) data processing pipeline comparison

Fig. 4

A An Upset plot of the cell barcodes that were generated by each pipeline after QC. Set size equals the total amount of BC generated by the pipeline. Intersection size representing the total amount of barcodes that overlap between the intersected set of pipelines. The bottom right panel shows which pipelines are being intersected. B UMAP after merging the data from the different pipelines together. Comparing cell expression content. Cells are color-coded according to the pipeline color used in Fig. 4A. C UMAP after merging the data from the different pipelines together. Cells are color coded by cell-type (also indicated at the right of Fig. 4C) after using the Azimuth cell annotation algorithm

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BMC Genomics

ISSN: 1471-2164

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