Fig. 1

Transcriptional profiling of M. intracellulare under oxidative and acidic stress conditions. The transcript data samples were analyzed from M. intracellulare cultures consisting of three groups as follows: Con (control): M. intracellulare cultures in pH 7.0 Sauton’s media, O (oxidative stress): M. intracellulare cultures in pH 7.0 Sauton’s media treated with 10 mM hydrogen oxide, A (acidic stress): M. intracellulare cultures in pH 4.5 Sauton’s media. Three biological replicates were used for analysis. A Multidimensional scaling plots of samples from transcript data samples. Three biological replicates have similar expression patterns in all experimental groups. B Cluster heatmap of differentially expressed genes (|fold change|≥ 2 and raw p < 0.05) in nine transcript data samples. C Numbers of differentially expressed genes in RNA-seq. The differentially expressed genes were defined as |fold change|≥ 2 and raw p < 0.05. D Venn diagram showing the shared genes that were significantly up- or down-regulated in M. intracellulare culture under oxidative and acidic stress conditions. Filter was set at |fold change|≥ 2 and raw p < 0.05