Table 1 Oligonucleotide sequences of primers used in qPCR validation
From: Prominent transcriptomic changes in Mycobacterium intracellulare under acidic and oxidative stress
Gene name | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) |
|---|---|---|
rpoB | ACCTCGGTGGTCAGGTAGTA | GGAAGGCAAGGCAATTCAGC |
narH | CAACCACAAGACGGGCAAAG | TCGGATCGTTAGGGTCCAGT |
narJ | CGCCACCATGTATCTGACGTA | GTGGCACGGTCAAGGTAAAC |
narI | GAAGATGAGCGACCACGTCT | AACCACAAACCGACCGTGTA |
narU | CGAGGAAGAACGCGATGTAGA | TACCGGATGATTTCGCGGAT |
groEL1 | GCTCTCCTTGCGTTCCTTGA | GAGGATCTGGCGATCGTGAC |
groEL2 | CTTCGCTGATGACCTGACCA | AAGGGCTACATCTCGGGCTA |
dinB | GTTGGGCGACATGGCATTAC | CCCGATCAGGAGTTGACGTT |
mmpS | GATGAACGGTTTGATGGCGT | GGGACCGCGAATCTGAACTA |
groES | TTACTTGGAGACGACAGCCA | GAAGGCGACACCGTCATCTA |