Fig. 8

The protein domain architecture of PcinCRN95 closely resembles that of CRNs functioning in cell death [28]. The domains in Phytophthora cinnamomi crinkling and necrosis effector protein 95 (PcinCRN95) are compared against the domains present in the CRN architecture of a CRN functioning in the induction of cell death, proposed by Zhang et al. [28]. . It was found that majority of the cell death inducing CRNs possessed a Ubl domain in the N-terminal followed by a NTPase and a restriction endonuclease (REase) superfamily in the C-terminal. The authors suggested that the Ubl domain could facilitate translocation inside the host nucleus, the toxicity function is specified by the REase domain, and the NTPase domain functions in regulating REase activity or affinity toward nucleic acids. There are notable similarities between the PcinCRN95 and the redefined CRN architecture, where both contain a ubiquitin-like (Ubl) domain (yellow), low complexity regions (LCR’s) (purple) and a nucleoside-triphosphatase (NTPase) domain (green) providing evidence for a potentially conserved functional role