Figure 3

RT-PCR results of selected genes. RT-PCR results of candidate genes derived without (A) and with (B) inclusion of additional selections rules described in materials and methods. The selection rules, which were applied for each of the analyzed are shown in (B) next to the images of the agarose gels. (C) Gapdh and Actb were used as loading controls. Water was used as a negative control for all reactions. With the exception of Gapdh and Actb (25 cycles), 28 amplification cycles were used.