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Table 2 General statistics for the subtractive libraries.

From: Discovering genes associated with dormancy in the monogonont rotifer Brachionus plicatilis

  sbs01 sbs02 sbs03 sbs04
# Reads* 1021 1189 1117 1203
# average read length (bp) 453 485 493 482
# singletons 421 541 429 198
# clusters 155 264 194 157
# putative transcripts 576 805 623 355
Avg cluster size 3.87 2.46 3.55 6.4
Largest cluster 81 15 59 63
# clusters with 2 ESTs 113 210 146 62
# clusters with 3 ESTs 19 29 17 25
# clusters with 4–5 ESTs 10 20 16 22
# clusters with 6–10 ESTs 6 2 7 22
# clusters with > 10 ESTs 7 3 8 26
Gene discovery 0.56 0.68 0.56 0.30
Gene diversity 0.41 0.46 0.38 0.16
# (%) with significant* SwissProt hits** 308 (53%) 415 (52%) 296 (48%) 100 (28%)
# (%) with significant* trembl hits** 328 (57%) 439 (55%) 309 (50%) 110 (31%)
# (%) with no hits 242 (42%) 363 (45%) 309 (50%) 243 (68%)
  1. * Reads that are submittable and > 100 bp. ** Threshold for Blastx significance = 1e-10. Gene discovery is defined as the number of different "genes" each library contributed, divided by library size. Gene Diversity is defined as the number of singletons in each library divided by library size [22].