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Table 3 Microarray data validation by qRT-PCR

From: Induction of lipid oxidation by polyunsaturated fatty acids of marine origin in small intestine of mice fed a high-fat diet

  

Microarray ratio

qRT-PCR ratio

Genes

Probeset ID

Pool

Pool

mean ratio (n = 10–11)

Acaa1a

1456011_x_at

1.67

2.00

2.52 *

Acaa1b

1424451_at

2.19

1.75

 

Acaa1a/Acaa1b

1416946_a_at

1.87

  
 

1416947_s_at

1.59

  

Acacb

1427052_at

6.9 a

n.d.c

3.91 *

Acox1

1416409_at

1.24

1.34

 
 

1416408_at

1.35

  

Acox2

1420673_a_at

2.91

1.97

 

Acox3

1420684_at

n.c.b

-1.23

 

Cpt1a

1434866_x_at

2.87

1.87

2.79 **

 

1438156_x_at

2.60

  

Cpt2

1416772_at

1.39

1.44

 

Ela2

1448281_a_at

2.04

4.26

 

Ela3b

1415884_at

2,64

7.89

 
 

1435611_x_at

4,20

  
 

1415883_a_at

4,38

  
 

1437326_x_at

4,72

  
 

1435012_x_at

5,17

  

H2-Q10

1425137_a_at

-8.22

-2.39

 

Hmgcs1

1433443_a_at

-1.82

-2.00

 
 

1433446_at

-1.57

  

Hmgcs2

1431833_a_at

3.61

3.97

 
 

1423858_a_at

4.56

  

Hsd3b2/6

1460232_s_at

2.50

n.d.

1.91 *

Hsd3b3

1427377_x_at

2.00

  

Mod1

1416632_at

2.16

2.60

 
 

1430307_a_at

2.13

  

Pck1

1423439_at

2.17

2.21

 
 

1439617_s_at

2.46

  

Pdk4

1417273_at

3.01

n.d.

3.24 *

Sqle

1415993_at

-2.14

-2.35

-1.55 *

  1. Genes are sorted alphabetically. In several cases gene family members were included for validation, although they did not show differential microarray gene expression. Data is represented by ratio of EPA&DHA/control diet. For qRT-PCR, data shown is obtained using a pool of samples (pool), and/or individual samples (EPA/DHA: n = 11, control diet: n = 10) and the mean ratio was calculated. a this probeset is non-expressed (absent) in control diet; ratio is 6.9; b n.c.: not changed. c n.d.: not analyzed; statistical significance was calculated using Student's t-test: * p < 0.05, ** p < 0.01.