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Table 1 PCR primer pairs for qRT-PCR expression analyses.

From: Population-specific gene expression in the plant pathogenic nematode Heterodera glycines exists prior to infection and during the onset of a resistant or susceptible reaction in the roots of the Glycine max genotype Peking

Afx probe set

Genbank ID

Gene

Primers

amplicon size (bp)

HgAffx.15612.1.S1_at

CB281382

Hg-unc-9

F: 5'AGCCTAATGATGATCGAAACACTC3'

135

   

R: 5'GAAACTGATCAGCACCGAAAATG3'

 

HgAffx.18723.1.S1_at

CA940457

Hg-unc-15

F: 5'TTGCGGAGCTGGAAATGACC3'

105

   

R: 5'GGCTGGCCTGCAACACCTT3'

 

HgAffx.21154.1.S1_at

CK394306

Hg-unc-27

F: 5'TGGAGGAGGAGAAGTACGACATCA3'

133

   

R: 5'TCATATTTGGACACTTTCTTCAGC3'

 

HgAffx.17035.1.S1_at

CB279321

Hg-unc-60B

F: 5'AGGCGACTTTGGGGCTGGAGAG3'

121

   

R: 5'ACGGCGGGGCAATTTTAGGTTC3'

 

HgAffx.13291.1.S1_at

CB374691

Hg-unc-97

F: 5'AGAGATCGGCGGAGCACTTTAC3'

106

   

R: 5'CAGCGCGGTCACCACTCTTTC3'

 

HgAffx.21881.2.S1_at

CK351699

Hg-unc-112

F: 5'GGGCCTCCACTTGGTCACTATTAT3'

118

   

R: 5'GTTCCGACATCCCTTCACTGCTC3'

 

HgAffx.11541.1.S1_at

CB934909

Hg-dys-1

F: 5'GGGCGATGACATGCGTGACTTC3'

150

   

R: 5'GCCTCTGTTTCCGCGTTCTGTGG3'

 
  1. For the PCR primers, the Genbank match for each unc homolog is provided. The amplicon length is provided in base pairs.