From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
Sample ID | Input (ng) | Mean library fragment size (bp) | Input (total molecules by mass) | ssDNA library (total molecules by UT-dPCR) | dPCR replicate CV | Library prep recovery % | Library type | Organism |
---|---|---|---|---|---|---|---|---|
TS-1 | 35 ng | 500 | 1.19 × 1011 | 2.61 × 107 | 7.07% | 0.022% | Shotgun | E. coli |
TS-2 | 25 ng | 500 | 8.52 × 1010 | 6.15 × 106 | 8.24% | 0.007% | Shotgun | E. coli |
TS-3 | 10 ng | 500 | 3.41 × 1010 | 4.20 × 106 | 7.71% | 0.013% | Shotgun | E. coli |
TS-4 | 5 ng | 500 | 1.70 × 1010 | 4.08 × 106 | 5.65% | 0.024% | Shotgun | E. coli |
TS-5 | 1 ng | 500 | 3.41 × 109 | 4.08 × 105 | 16.84% | 0.012% | Shotgun | E. coli |
TS-6 | 500 pg | 500 | 1.70 × 109 | 2.31 × 105 | 5.58% | 0.014% | Shotgun | E. coli |
TS-7 | 35 ng | 466 | 1.27 × 1011 | 2.67 × 107 | 8.55% | 0.023% | Amplicon | E. coli |
TS-8 | 25 ng | 466 | 9.10 × 1010 | 2.11 × 107 | 8.50% | 0.025% | Amplicon | E. coli |
TS-9 | 10 ng | 466 | 3.64 × 1010 | 2.15 × 106 | 4.90% | 0.006% | Amplicon | E. coli |
TS-10 | 5 ng | 466 | 1.82 × 1010 | 8.49 × 105 | 7.10% | 0.005% | Amplicon | E. coli |
TS-11 | 1 ng | 466 | 3.64 × 109 | 8.67 × 104 | 21.90% | 0.002% | Amplicon | E. coli |
TS-12 | 500 pg | 466 | 1.82 × 109 | 1.31 × 105 | 7.60% | 0.007% | Amplicon | E. coli |
IgG | ~1.2 nga | 180 | 1.18 × 1011 | 3.24 × 106 | 17.61% | 0.003% | Shotgun | M. musclus |
K27-1 | ~1.2 nga | 180 | 1.01 × 1011 | 1.87 × 106 | 9.40% | 0.002% | Shotgun | M. musclus |
K27-2 | ~1.2 nga | 180 | 8.41 × 1010 | 1.49 × 106 | 5.65% | 0.002% | Shotgun | M. musclus |
Ace | 723 ng | 550 | 2.26 × 1012 | 3.63 × 108 | 6.10% | 0.016% | Shotgun | A. longum |
pX | 4.9 ngb | 180 | 9.26 × 1010 | 9.06 × 106 | 4.60% | 0.010% | Shotgun | H. sapien |