From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
Sample ID | Organism | Library Type | input (ng) | Proportion of library sequenced | Raw bases (Mbp) | Number of reads | Average read length (bp) | % mapping to template/assembling* |
---|---|---|---|---|---|---|---|---|
TS-5 | E. coli | Shotgun | 1.0 ng | 1.0 | 6.0 | 28730 | 210.5 | 69.9% |
TS-6 | E. coli | Shotgun | 0.5 ng | 1.0 | 5.7 | 26812 | 212.5 | 69.9% |
TS-11 | E. coli | Amplicon | 1.0 ng | 0.5 | 10.9 | 43217 | 252.5 | 99.2% |
TS-12 | E. coli | Amplicon | 0.5 ng | 0.5 | 14.0 | 55206 | 253.6 | 99.0% |
IgG | M. Musclus | Shotgun | ~1.2 ng | 1.0 | 3.8 | 27712 | 139.8 | 10.1% |
K27-1 | M. Musclus | Shotgun | ~1.2 ng | 1.0 | 4.1 | 27701 | 147.7 | 35.3% |
K27-2 | M. Musclus | Shotgun | ~1.2 ng | 1.0 | 7.1 | 42829 | 166.0 | 21.0% |
pX | H. Sapien | Shotgun | 4.9 ng | 0.21 | 42 | 244010 | 172.6 | 64.6% |
Ace | A. longum | Shotgun | 723 ng | 0.005 | 67 | 278181 | 240.9 | 98.8%* |