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Table 3 454 FLX trace library sequence results

From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing

Sample ID

Organism

Library Type

input (ng)

Proportion of library sequenced

Raw bases (Mbp)

Number of reads

Average read length (bp)

% mapping to template/assembling*

TS-5

E. coli

Shotgun

1.0 ng

1.0

6.0

28730

210.5

69.9%

TS-6

E. coli

Shotgun

0.5 ng

1.0

5.7

26812

212.5

69.9%

TS-11

E. coli

Amplicon

1.0 ng

0.5

10.9

43217

252.5

99.2%

TS-12

E. coli

Amplicon

0.5 ng

0.5

14.0

55206

253.6

99.0%

IgG

M. Musclus

Shotgun

~1.2 ng

1.0

3.8

27712

139.8

10.1%

K27-1

M. Musclus

Shotgun

~1.2 ng

1.0

4.1

27701

147.7

35.3%

K27-2

M. Musclus

Shotgun

~1.2 ng

1.0

7.1

42829

166.0

21.0%

pX

H. Sapien

Shotgun

4.9 ng

0.21

42

244010

172.6

64.6%

Ace

A. longum

Shotgun

723 ng

0.005

67

278181

240.9

98.8%*