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Table 3 454 FLX trace library sequence results

From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing

Sample ID Organism Library Type input (ng) Proportion of library sequenced Raw bases (Mbp) Number of reads Average read length (bp) % mapping to template/assembling*
TS-5 E. coli Shotgun 1.0 ng 1.0 6.0 28730 210.5 69.9%
TS-6 E. coli Shotgun 0.5 ng 1.0 5.7 26812 212.5 69.9%
TS-11 E. coli Amplicon 1.0 ng 0.5 10.9 43217 252.5 99.2%
TS-12 E. coli Amplicon 0.5 ng 0.5 14.0 55206 253.6 99.0%
IgG M. Musclus Shotgun ~1.2 ng 1.0 3.8 27712 139.8 10.1%
K27-1 M. Musclus Shotgun ~1.2 ng 1.0 4.1 27701 147.7 35.3%
K27-2 M. Musclus Shotgun ~1.2 ng 1.0 7.1 42829 166.0 21.0%
pX H. Sapien Shotgun 4.9 ng 0.21 42 244010 172.6 64.6%
Ace A. longum Shotgun 723 ng 0.005 67 278181 240.9 98.8%*