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Table 4 Solexa trace library generation & sequence results

From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing

Solexa Libraries Input (ng)a DNA Library (total molecules by UT-dPCR)*/ul Average number of clusters generated per tile Total number of reads % mapping to Human reference (hg 18)
Plasma DNA Sample 1 3.2 1.07 × 1011 115998 11599833 51.5%
Plasma DNA Sample 2 3.6 7.88 × 1010 114548 11454876 52.7%
Plasma DNA Sample 3 2.7 7.17 × 1010 118516 11851612 56.1%
Plasma DNA Sample 4 2.6 6.03 × 1010 150414 15041417 49.7%
Plasma DNA Sample 5 5.6 7.17 × 1010 119104 11910483 56.1%
Plasma DNA Sample 6 2.4 7.23 × 1010 120974 12097478 55.4%
Whole Blood DNA Sample 2.1 6.30 × 1010 151201 15120171 50.5%
  1. a Quantified by digital PCR with human specific primers on a unique locus taking 6.6 pg per cell.