From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing
Solexa Libraries | Input (ng)a | DNA Library (total molecules by UT-dPCR)*/ul | Average number of clusters generated per tile | Total number of reads | % mapping to Human reference (hg 18) |
---|---|---|---|---|---|
Plasma DNA Sample 1 | 3.2 | 1.07 × 1011 | 115998 | 11599833 | 51.5% |
Plasma DNA Sample 2 | 3.6 | 7.88 × 1010 | 114548 | 11454876 | 52.7% |
Plasma DNA Sample 3 | 2.7 | 7.17 × 1010 | 118516 | 11851612 | 56.1% |
Plasma DNA Sample 4 | 2.6 | 6.03 × 1010 | 150414 | 15041417 | 49.7% |
Plasma DNA Sample 5 | 5.6 | 7.17 × 1010 | 119104 | 11910483 | 56.1% |
Plasma DNA Sample 6 | 2.4 | 7.23 × 1010 | 120974 | 12097478 | 55.4% |
Whole Blood DNA Sample | 2.1 | 6.30 × 1010 | 151201 | 15120171 | 50.5% |