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Table 6 Primer/probe list for UT-quantitative PCR and UT-digital PCR

From: Digital PCR provides sensitive and absolute calibration for high throughput sequencing

Primers for Standard 454 libraries:
Forward: 5'-CCATCTCATCCCTGCGTGTC-3'
Reverse: 5'-CCTATCCCCTGTGTGCCTTG-3'
UTBP-1: 5'-GGCGGCGACCATCTCATCCCTGCGTGTC-3'
Primers for 454 MID/Paired end libraries:
Forward: 5'-GCCTCCCTCGCGCCATCAG-3'
Reverse: 5'-GCCTTGCCAGCCCGCTCAG-3'
UTBP-2: 5'-GGCGGCGAGCCTCCCTCGCGCCATCAG-3'
Primers for Solexa libraries:
Forward: 5'-ACACTCTTTCCCTACACGA-3'
Reverse: 5'-CAAGCAGAAGACGGCATA-3'
UTBP-3: 5'-GGCGGCGAACACTCTTTCCCTACACGA-3'
Universal probe sequence:
UPL#149 5'-CCGCCGCT-3'