Figure 4

Real Time PCR (qRT-PCR) analysis of drought and ABA-responsive gene expression. The y axis refers to the relative expression ratio between target mRNA versus the reference mRNA (polyubiquitin SCCCST2001G02.g; GAPDH Gene ID: 542367; UBE2 SCBGLR1002D06.g) in sugarcane plants treated with ABA for 12 h or drought conditions for 24, 72 or 120 h. The reactions for the target mRNA and reference mRNA were carried out in parallel and each reaction was performed in triplicates. Error bars were calculated as described previously [31]. The transcript levels for the reference genes were verified to not vary in response to the treatments. The values of P correspond to the probability Pr (Treated>Control) and Pr (Treated<Control) for up- and down-regulated genes, respectively. The SAS was considered differentially expressed when P ≥ 0.95.