Table 1 Comparison of the efficiency of microsatellite enrichment from unnormalized and normalized cDNA libraries of Asian seabass
From: A simple and efficient method for isolating polymorphic microsatellites from cDNA
cDNA | Enriched for | No clones a | No MS clonesb | No singlteons c | No clusters d | No unique microsatellites | % |
|---|---|---|---|---|---|---|---|
Unnormalized | (CA)n | 192 | 80 | 11 | 8 | 19 | 9.9 |
(GA)n | 192 | 40 | 9 | 5 | 14 | 7.3 | |
Normalized | (CA)n | 192 | 88 | 41 | 10 | 51 | 26.5 |
(GA)n | 192 | 51 | 35 | 5 | 40 | 20.8 |