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Table 1 Comparison of the efficiency of microsatellite enrichment from unnormalized and normalized cDNA libraries of Asian seabass

From: A simple and efficient method for isolating polymorphic microsatellites from cDNA

cDNA Enriched for No clones a No MS clonesb No singlteons c No clusters d No unique microsatellites %
Unnormalized (CA)n 192 80 11 8 19 9.9
  (GA)n 192 40 9 5 14 7.3
Normalized (CA)n 192 88 41 10 51 26.5
  (GA)n 192 51 35 5 40 20.8
  1. a: Number of clones sequenced; b: Number of clones containing microsatellites; c: Number of singletons containing microsatellites; d: number of clusters containing microsatellites, and %: percentage of clones containing unique microsatellites