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Figure 1 | BMC Genomics

Figure 1

From: Norepinephrine transport-mediated gene expression in noradrenergic neurogenesis

Figure 1

Time course of expression of NET mRNA and NET function in cultured mouse neural crest cells. (A) Semi-quantitative RT-PCR of NET mRNA on culture days 4 – 8 (upper) and Hprt (lower) at 28, 30 and 32 cycles each. Based on these results NET was subsequently amplified at 30 cycles. (B) Ratio Net/Hprt in triplicate during culture days 4 – 9 at 30 cycles of amplification. Data are expressed as average of three samples; error bars represent standard deviation. (C) Morphology of 3H-NE uptake-positive cells at day 3 of culture resembles immature neural crest cells. (D) At day 7 of culture, many 3H-NE uptake-positive cells have the morphology of mature sympathetic neuroblasts (arrow); NET uptake positive cells with undifferentiated morphology were still present (arrowhead). These observations indicated that at day 7, culture contain NE uptake-positive progenitors as well as neuroblasts with a functional NET. They are multipolar and extend long processes. Bar, (C, D) 100 μm.

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