Figure 2

Altered morphology and reduced numbers of DBH-immunoreactive cells in day 7 NETKO neural crest cultures. (A) NETKO neural crest cells have no or short processes only (e.g., arrow). (B) By contrast, wild type DBH-positive cells have long processes (e.g., arrow). (C) Quantification of the number of DBH-immunoreactive cells expressed in day 7 wild type and NETKO neural crest cultures. Approximately half the number of DBH-positive cells is expressed in NETKO cultures compared to wild type cultures. Bar, (A, B) 100 μm.