Figure 3

Co-localization of CART and DBH immunoreactivities in wild type and NETKO neural crest cells, superior cervical ganglion and locus ceruleus. (A, A', B, B'), NETKO and wild type neural crest cultures, respectively, at day 7. (C, C', D, D'), superior cervical ganglion of 10 week-old mice; (E, E', F, F'), Locus ceruleus from 10-week old wild type and NETKO mice. Wild type cells express CART at low levels. DBH and CART immunoreactivities co-localize in all three tissues. CART and DBH immunoreactivity appear somewhat brighter in NETKO tissue than in wild type tissues, suggesting increase in expression not only at the RNA but also at the protein level. It needs to be noted that immunofluorescence was not quantified. Bar, (A – F') 100 μm.