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Table 1 Assessment of the cDNA library normalization by sequence count

From: A transcriptional sketch of a primary human breast cancer by 454 deep sequencing

Reference Gene1 454 Reads mapped to the genome (194,806) UniGene ESTs
(39,700)
Probability of differential expression between the libraries
ACTB 11 187 Prob > 0.999
GAPDH 31 225 Prob > 0.999
HPRT1 7 0 0.5 < Prob < 0.6
  1. 1 ACTB and GAPDH are abundantly expressed housekeeping genes, while HPRT1 is expressed at low levels.