Table 2 Primary classification of the 454 sequencing reads
From: A transcriptional sketch of a primary human breast cancer by 454 deep sequencing
Set Description | Number of reads |
|---|---|
Total (unfiltered) | 251,262 |
Mapping to the genome, 70% coverage, high stringency | 194,806 |
Subset with a single match on the genome at 98% identity and 98% coverage (98.98.1 dataset) 1 | 132,113 |
Subset with a single match on the genome and 100% coverage of the alignment2 | 114,427 |
Subset of 98.98.1 dataset matching with max 6 errors (mismacthes + indels) and 90% coverage on UCSC all_mrna and RefSeq – canonical transcripts dataset | 59,632 |
Subset of 98.98.1 dataset matching inside an UCSC Known Gene (Intragenic dataset, intronic + exonic transcripts) | 118,840 |
Matching with max 6 errors (mismatches + indels) and 90% coverage to the Human ORESTES EST dataset (764,587 sequences) | 68,396 |