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Table 2 The thirty most highly expressed genes in macular RPE identified in six different human donors, sorted by intensity in descending order.

From: Functional annotation of the human retinal pigment epithelium transcriptome

gene symbol Genbank ID mean intensity μint perc CV perc gene name relevant function
KIAA0241 AA205569 99 98 KIAA0241  
AI003379 AI003379 99 98 Transcribed locus  
ACN9 NM_020186 99 98 ACN9 homolog gluconeogenesis [25]
MT1A BG191659 99 98 Metallothionein 1A protection against reactive oxygen species[22]
SLC17A7 NM_020309 99 98 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 glutamate transporter, expressed in RPE[14, 15]
TP53 NM_000546 99 98 Tumor protein p53 protection against reactive oxygen species[23]
ELF2 NM_006874 99 98 E74-like factor 2 (ets domain transcription factor)  
AI272368 AI272368 99 95 cDNA clone  
AA807363 AA807363 99 79 cDNA clone  
SERPINB5 NM_002639 99 98 Serpin peptidase inhibitor, clade B, member 5  
MCM7 NM_005916 99 97 minichromosome maintenance deficient 7  
SLC1A2 NM_004171 99 96 Solute carrier family 1 member 2 glutamate transporter[1]
HBB NM_000518 99 99 Hemoglobin, beta  
PTGDS NM_000954 99 94 Prostaglandin D2 synthase 21 kDa released from RPE during rod phagocytosis [17, 19]
T26536 T26536 99 75 cDNA clone  
EEF1A1 NM_001402 99 80 Eukaryotic translation elongation factor 1 alpha 1  
TTR NM_000371 99 80 Transthyretin (prealbumin, amyloidosis type I) maintains normal levels of retinol and retinol binding proteins in plasma [14, 20]
BE260168 BE260168 99 90 cDNA clone  
CST3 NM_000099 99 92 Cystatin C associated with AMD[16, 18]
ZNF503 NM_032772 99 95 Zinc finger protein 503  
BG190000 BG190000 99 94 cDNA clone  
BE262306 BE262306 99 80 cDNA clone  
HSP90B1 NM_003299 99 94 Heat shock protein 90 kDa beta (Grp94), member 1 [21]
GNGT1 NM_021955 99 90 Guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1  
RPL3 NM_000967 99 20 Ribosomal protein L3  
RPL41 NM_021104 99 23 Ribosomal protein L41  
AI857840 AI857840 99 77 cDNA clone  
PCSK7 NM_004716 99 90 Proprotein convertase subtilisin/kexin type 7  
AL521537 AL521537 99 75 cDNA clone  
CLU NM_001831 99 42 Clusterin member of complement system [24]
  1. Among these genes we identified five genes with known expression in the RPE (SLC17A7[14], CST3[18], TTR[14], HSP90B1[21] and PTGDS[17]). The SLC17A7 gene is a glutamate transporter, like the SLC1A2 gene which is also in the top 30 highly expressed genes. The CST3 gene was previously suggested to have an association with AMD[16, 17]. The list also contains two genes with a role in the protection against oxidative stress (MT1A[22], TP53[23]). The GNGT1 gene, expressed in photoreceptors[30], suggests the inevitable presence of photoreceptor contamination. Perc: percentile.