Skip to main content

Table 1 Oligonucleotide probe sequences for single-nucleotide polymorphism (SNP) markers

From: Two-stage genome-wide association study identifies integrin beta 5 as having potential role in bull fertility

NCBI SNP ID Locus Primers and detection probes
Rs29016875 C/T Forward primer: 5'-GTCTGGTATTCCCATCTCTTTCAGA-3'
Reverse primer: 5'-TTACTGATTGAAGGGCAACTGTGT-3'
Probe 1: 5'-6FAM-TTTTCCACAGT TTATTGTG-3'
Probe 2: 5'-VIC-TTTTCCACAGC TTATTG-3'
Rs29015574 C/T Forward primer: 5'-ACTCTGTCTCTGAGATTCGATTCAGT-3'
Reverse primer: 5'-CTGAAATCTTTCATTCCCTAGCTGATG-3'
Probe 1: 5'-6FAM-CTGAAAACTCTA TCTCTG-3'
Probe 2: 5'-VIC-CTGAAAACTCTG TCTCTG-3'
Rs29024867 G/C Forward primer: 5'-TGGAGGAGTTCTTTAATGCTTATAAATG-3'
Reverse primer: 5'-GGAGGCACAAAATAGTTAACAGACATC-3'
Probe 1: 5'-6FAM-CTAAACC GATTTGTAATC-3'
Probe 2: 5'-VIC-CTAAACG GATTTGTAATC-3'
Rs41257187 C/T Forward primer: 5'-CGAAATGGCTTCAAACCCTCTGTA-3'
Reverse primer: 5'-TGTTGAAGGTGAAATCCACAGTGT-3'
Probe 1: 5'-6FAM-CAGAAAGCCT ATCTCC-3'
Probe 2: 5'-VIC-AGAAAGCCC ATCTCC-3'