Table 2 Primers used for Real Time PCR gene expression analysis of HMGN3a, the housekeeping gene GAPDH, and SMARCAL1.
From: Functional genomics of HMGN3a and SMARCAL1 in early mammalian embryogenesis
Gene | Primer sequence and position (5' - 3') | Product size (bp) | Accession Number |
|---|---|---|---|
HMGN3a_F | GTTCCAGCCCGTTGCTTTAC (22 – 42) | 355 | NM_001034504.1 |
HMGN3a_R | GACCATTCATTCTCCCTCGTTAC (376 – 399) |  |  |
GAPDH_F | TGCTGGTGCTGAGTATGTGGT (333 – 354) | 295 | XM_865742 |
GAPDH_R | AGTCTTCTGGGTGGCAGTGAT (627–648) |  |  |
SMARCAL1_F | CCATCTGCATAGCGGCCTAT (1397–1416) | 141 | NM_176666 |
SMARCAL1_R | CGGTTACCACGACGTTGATGT (1537–1517) |  |  |