Table 2 The oligonucleotides used for quantitative PCR to measure relative transcript abundance of ancient bovine PAGs during pregnancy.
PAG | Sequence | Primer location (bp) | Amplicon size (bp) |
|---|---|---|---|
BoPAG-2 | F: 5'-GTAGGCTCGCCTATCACCATCTTC-3' | 360 | 289 |
R: 5'-CCTCTGGCTTGTTTGTGTTCAAGTAG-3' | 649 | ||
BoPAG-8 | F: 5'-CCTATCCTGAATGACGAGCAA-3' | 886 | 128 |
R: 5'-CCTTTCAGAAAACCTCTGGATG-3' | 1014 | ||
BoPAG-10 | F: 5'-TTGAGCAGTCAGAAAGAGAACG-3' | 631 | 136 |
R: 5'-TTCATGGAGATGCTGTCTATGTTT-3' | 767 | ||
BoPAG-11 | F: 5'-CGGTTCCGAGTACATGGTTT-3' | 885 | 114 |
R: 5'-AGAAATCTTTTGGATGTAGGCTTC-3' | 999 | ||
BoPAG-12 | F: 5'-ACACACCAGCCTATTAGCATCTCC-3' | 361 | 266 |
R: 5'-CGGCTGGCATGTGTTCAAGTAG-3' | 627 | ||
YHWAG | F: 5'-AGCACATGCAGCCCACTC-3' | 488 | 120 |
R: 5'-TCGTCGAAGGCGGTCTTG-3' | 608 |