Table 2 Primers used for WC1 transcript amplification and for cDNA clone sequencing reactions
Primer name | Sequence (5'-3') |
|---|---|
Primers used for gene amplification: | |
WC1atg-for | ATGGCTCTGGGCAGACACCTCTC |
WC1full-forA | GATGGTTACAGGTGGACATTGAAGG |
WC1group1,2-rev | TCAYGAGAAAGTCAYTGKGGATG |
WC1group3-rev | CTACATGGTGCTAAGCTCCACATC |
WC1group4-rev | TCATCTCCTAGTTAATACAGCATC |
SRCR1-rev | TGCACAGATGACCTGGGCAGTGG |
Primers used for sequencing reactions: | |
WC1seq1-for | CTCAACCTACAGGCTCTG |
WC1seq2-for | CAGGTGGAGATGAACATTTC |
WC1seq3-for | CACGACTGCAGACACAAGC |
WC1seq4-for | GAAGACATCACTGTGTCCGTG |
WC1seq5-for | GAGAAGCCCTCAATGCCAC |
WC1seq6-for | CATGGAAGACATCACTGTG |
WC1seq7-for | GCTACACAGATGGAGAGCAG |
WC1seq8-for | GGCTCATGACAAACGGCTCCTCTCAG |
WC1seq1-rev | GAGCTTCTCTCTGTCTGTGCAG |
WC1seq2-rev | GCAGCACAGTCCCACAG |
WC1seq3-rev | GCCCTTCCCCAAAGTGAGCTG |
WC1seq4-rev | CTGGTCAAGGATCTCCACTCTC |
WC1seq5-rev | GATCCTGCCCCAAAGTGAGC |
WC1seq6-rev | GTGTGAAGTTTCCATCAGAC |
WC1seq7-rev | GCAGTAGGGAGAACTCTC |