Figure 11

Sequence comparison of regulatory elements in the bovine, human and murine TRB loci. (A) Alignment of the human, bovine and murine minimal enhancer sequences. Protein binding sequences in the human (Tβ) and murine (βE) minimal Eβ region are indicated by arrows. (B) Alignment of the murine PDβ1 with the bovine sequence immediately upstream of the TRBD1 gene. The position of the TRBD1 gene and the flanking RSs are indicated. Shaded areas indicate the location of defined transcription regulation elements as defined in the key. Identity is shown by dashes and gaps by dots. (C) Sequence logos for the conserved TGAxxTCA CRE motif and adjacent bases found in the promoter regions (at ~80–120 bp upstream) of 57 bovine, 52 human and 23 murine TRBV genes. The location of conserved TGA and TCA are enclosed in boxes. The height of the letters is correlated to their conservation at each location.