Skip to main content

Table 2 Differential expression of myostatin targets genes confirmed by qPCR and by western-blot experiments in MSTN-null mice vs their control littermates.

From: Molecular profiles of Quadriceps muscle in myostatin-null mice reveal PI3K and apoptotic pathways as myostatin targets

 

Proteomics

Transcriptomics

qPCR

Western-Blot

H-FABP

-2.1

  

-9.1

MYBPH

3.1

  

1.4

MyHCII

   

4.9

Syntrophin A

1.5

  

2.8

p-Akt

   

2.2

GSK-3β

 

-1.9

-2.4

 

GSK-3β(ser9)/GSK-3β

   

1.7 t

PI3KR3

 

1.1

1.8

 

DJ-1

1.5

 

1.7

2.2

PINK1

 

1.9

 

3.4

PTEN

  

-1.6 t

-1.6

Mortalin

-1.5

 

-1.4

-2.1

TCTP

2.7

  

1.6

14-3-3E

1.8

 

1.4

1.7

mfgf6

  

1.9

 
  1. Results are expressed as Fold Changes and all the differences have been declared significant (P < 0.05) except for some tendencies (t: P < 0.10).
  2. H-FABP: Heart-Fatty acid binding protein H; MyBP-H: Myosin binding protein H; MyHCII: fast myosin heavy chain isoform; pAkt: phosphorylated Akt; GSK-3β: Glycogen synthase kinase 3β; GSK-3β (ser9): Glycogen synthase kinase 3β (phosphorylated at serine 9); PI3KR3: Phosphatidylinositol 3-kinase regulatory subunit; DJ-1: cancer- and Parkinson's disease-associated protein (park7); PINK1: PTEN-induced putative kinase 1; PTEN: tumour-suppressor phosphatase with tensin homology. TCTP: Translationally controlled tumor protein; 14-3-3E: 14-3-3ε protein; mfgf6: murine Fibroblast growth factor 6.