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Table 1 GeneChip Array performance metrics

From: Gene expression profiling of whole blood: Comparison of target preparation methods for accurate and reproducible microarray analysis

Sample

Bg

% P

Mean Signal

Actin Intensity

Actin 3'/5' Ratio

GAPDH Intensity

GAPDH 3'/5' Ratio

METHOD 1

       

A

54.3

48.3%

95.5

751.4

7.6

410.4

5.9

C

50.3

53.1%

123.3

3437.5

1.4

988.1

1.8

D

47.8

52.2%

107.5

3692.0

1.1

1216.7

1.1

A frozen

49.0

51.2%

95.0

2641.6

1.4

1073.3

1.2

C frozen

48.7

54.0%

117.7

3618.0

1.2

1324.7

1.2

D frozen

49.7

51.3%

98.6

2933.7

1.1

1005.6

1.5

Average*

50.1

52.4%

106.3

2845.7

1.2

1003.1

1.4

METHOD 2

       

A

58.2

58.9%

270.4

8145.6

1.5

3492.0

0.9

C

52.7

58.7%

220.0

6812.9

1.5

2742.4

1.0

D

62.2

55.9%

179.7

6953.0

1.1

2697.9

1.0

A frozen

53.1

57.2%

177.2

5616.2

1.5

2336.8

1.1

C frozen

56.1

58.5%

195.4

6458.0

1.2

2580.7

0.9

D frozen

55.2

58.3%

196.8

6952.1

1.2

3016.1

1.0

Average

56.2

57.9%

206.6

6823.0

1.3

2811.0

1.0

METHOD 3

       

A

53.2

56.9%

155.8

2014.2

3.2

680.1

8.3

C

51.3

56.1%

145.1

4514.5

1.1

689.9

4.3

D

51.7

55.7%

144.5

3492.7

1.7

676.4

8.0

A frozen

46.5

56.6%

136.7

3400.5

2.0

1442.0

1.4

C frozen

49.5

55.1%

129.7

1491.0

3.9

583.8

8.9

D frozen

45.6

56.3%

132.2

3598.3

1.2

678.7

2.9

Average

49.6

56.1%

140.7

3085.2

2.2

791.8

5.6

METHOD 4

       

A

41.8

55.8%

97.0

2057.3

7.2

301.5

1.5

C

30.9

53.4%

63.6

1658.4

10.7

161.3

1.6

D

31.2

55.7%

82.4

2039.6

10.2

259.4

1.9

A frozen

30.8

53.8%

65.2

1577.0

15.5

183.4

2.9

C frozen

34.8

53.3%

73.2

1753.5

11.1

190.2

2.1

D frozen

37.9

53.3%

79.3

1838.4

10.6

256.3

1.8

Average

34.6

54.2%

76.8

1820.7

10.9

225.4

2.0

  1. Microarray targets prepared from donors A, C, and D were hybridized to the GeneChip Human Focus Array. Overall performance values determined with unscaled MAS 5.0 analysis of each array are listed. Bold font indicates metric values that fall below empirical quality control standards established in our laboratory. All arrays passed post-hybridization visual inspection. *Average values for Method 1 do not include sample A which was flagged as a low performance outlier.