Sample | Bg | % P | Mean Signal | Actin Intensity | Actin 3'/5' Ratio | GAPDH Intensity | GAPDH 3'/5' Ratio |
---|---|---|---|---|---|---|---|
METHOD 1 | Â | Â | Â | Â | Â | Â | Â |
A | 54.3 | 48.3% | 95.5 | 751.4 | 7.6 | 410.4 | 5.9 |
C | 50.3 | 53.1% | 123.3 | 3437.5 | 1.4 | 988.1 | 1.8 |
D | 47.8 | 52.2% | 107.5 | 3692.0 | 1.1 | 1216.7 | 1.1 |
A frozen | 49.0 | 51.2% | 95.0 | 2641.6 | 1.4 | 1073.3 | 1.2 |
C frozen | 48.7 | 54.0% | 117.7 | 3618.0 | 1.2 | 1324.7 | 1.2 |
D frozen | 49.7 | 51.3% | 98.6 | 2933.7 | 1.1 | 1005.6 | 1.5 |
Average* | 50.1 | 52.4% | 106.3 | 2845.7 | 1.2 | 1003.1 | 1.4 |
METHOD 2 | Â | Â | Â | Â | Â | Â | Â |
A | 58.2 | 58.9% | 270.4 | 8145.6 | 1.5 | 3492.0 | 0.9 |
C | 52.7 | 58.7% | 220.0 | 6812.9 | 1.5 | 2742.4 | 1.0 |
D | 62.2 | 55.9% | 179.7 | 6953.0 | 1.1 | 2697.9 | 1.0 |
A frozen | 53.1 | 57.2% | 177.2 | 5616.2 | 1.5 | 2336.8 | 1.1 |
C frozen | 56.1 | 58.5% | 195.4 | 6458.0 | 1.2 | 2580.7 | 0.9 |
D frozen | 55.2 | 58.3% | 196.8 | 6952.1 | 1.2 | 3016.1 | 1.0 |
Average | 56.2 | 57.9% | 206.6 | 6823.0 | 1.3 | 2811.0 | 1.0 |
METHOD 3 | Â | Â | Â | Â | Â | Â | Â |
A | 53.2 | 56.9% | 155.8 | 2014.2 | 3.2 | 680.1 | 8.3 |
C | 51.3 | 56.1% | 145.1 | 4514.5 | 1.1 | 689.9 | 4.3 |
D | 51.7 | 55.7% | 144.5 | 3492.7 | 1.7 | 676.4 | 8.0 |
A frozen | 46.5 | 56.6% | 136.7 | 3400.5 | 2.0 | 1442.0 | 1.4 |
C frozen | 49.5 | 55.1% | 129.7 | 1491.0 | 3.9 | 583.8 | 8.9 |
D frozen | 45.6 | 56.3% | 132.2 | 3598.3 | 1.2 | 678.7 | 2.9 |
Average | 49.6 | 56.1% | 140.7 | 3085.2 | 2.2 | 791.8 | 5.6 |
METHOD 4 | Â | Â | Â | Â | Â | Â | Â |
A | 41.8 | 55.8% | 97.0 | 2057.3 | 7.2 | 301.5 | 1.5 |
C | 30.9 | 53.4% | 63.6 | 1658.4 | 10.7 | 161.3 | 1.6 |
D | 31.2 | 55.7% | 82.4 | 2039.6 | 10.2 | 259.4 | 1.9 |
A frozen | 30.8 | 53.8% | 65.2 | 1577.0 | 15.5 | 183.4 | 2.9 |
C frozen | 34.8 | 53.3% | 73.2 | 1753.5 | 11.1 | 190.2 | 2.1 |
D frozen | 37.9 | 53.3% | 79.3 | 1838.4 | 10.6 | 256.3 | 1.8 |
Average | 34.6 | 54.2% | 76.8 | 1820.7 | 10.9 | 225.4 | 2.0 |