|
Sample
|
Bg
|
% P
|
Mean Signal
|
Actin Intensity
|
Actin 3'/5' Ratio
|
GAPDH Intensity
|
GAPDH 3'/5' Ratio
|
|---|
|
METHOD 1
| | | | | | | |
|
A
|
54.3
|
48.3%
|
95.5
|
751.4
|
7.6
|
410.4
|
5.9
|
|
C
|
50.3
|
53.1%
|
123.3
|
3437.5
|
1.4
|
988.1
|
1.8
|
|
D
|
47.8
|
52.2%
|
107.5
|
3692.0
|
1.1
|
1216.7
|
1.1
|
|
A frozen
|
49.0
|
51.2%
|
95.0
|
2641.6
|
1.4
|
1073.3
|
1.2
|
|
C frozen
|
48.7
|
54.0%
|
117.7
|
3618.0
|
1.2
|
1324.7
|
1.2
|
|
D frozen
|
49.7
|
51.3%
|
98.6
|
2933.7
|
1.1
|
1005.6
|
1.5
|
|
Average*
|
50.1
|
52.4%
|
106.3
|
2845.7
|
1.2
|
1003.1
|
1.4
|
|
METHOD 2
| | | | | | | |
|
A
|
58.2
|
58.9%
|
270.4
|
8145.6
|
1.5
|
3492.0
|
0.9
|
|
C
|
52.7
|
58.7%
|
220.0
|
6812.9
|
1.5
|
2742.4
|
1.0
|
|
D
|
62.2
|
55.9%
|
179.7
|
6953.0
|
1.1
|
2697.9
|
1.0
|
|
A frozen
|
53.1
|
57.2%
|
177.2
|
5616.2
|
1.5
|
2336.8
|
1.1
|
|
C frozen
|
56.1
|
58.5%
|
195.4
|
6458.0
|
1.2
|
2580.7
|
0.9
|
|
D frozen
|
55.2
|
58.3%
|
196.8
|
6952.1
|
1.2
|
3016.1
|
1.0
|
|
Average
|
56.2
|
57.9%
|
206.6
|
6823.0
|
1.3
|
2811.0
|
1.0
|
|
METHOD 3
| | | | | | | |
|
A
|
53.2
|
56.9%
|
155.8
|
2014.2
|
3.2
|
680.1
|
8.3
|
|
C
|
51.3
|
56.1%
|
145.1
|
4514.5
|
1.1
|
689.9
|
4.3
|
|
D
|
51.7
|
55.7%
|
144.5
|
3492.7
|
1.7
|
676.4
|
8.0
|
|
A frozen
|
46.5
|
56.6%
|
136.7
|
3400.5
|
2.0
|
1442.0
|
1.4
|
|
C frozen
|
49.5
|
55.1%
|
129.7
|
1491.0
|
3.9
|
583.8
|
8.9
|
|
D frozen
|
45.6
|
56.3%
|
132.2
|
3598.3
|
1.2
|
678.7
|
2.9
|
|
Average
|
49.6
|
56.1%
|
140.7
|
3085.2
|
2.2
|
791.8
|
5.6
|
|
METHOD 4
| | | | | | | |
|
A
|
41.8
|
55.8%
|
97.0
|
2057.3
|
7.2
|
301.5
|
1.5
|
|
C
|
30.9
|
53.4%
|
63.6
|
1658.4
|
10.7
|
161.3
|
1.6
|
|
D
|
31.2
|
55.7%
|
82.4
|
2039.6
|
10.2
|
259.4
|
1.9
|
|
A frozen
|
30.8
|
53.8%
|
65.2
|
1577.0
|
15.5
|
183.4
|
2.9
|
|
C frozen
|
34.8
|
53.3%
|
73.2
|
1753.5
|
11.1
|
190.2
|
2.1
|
|
D frozen
|
37.9
|
53.3%
|
79.3
|
1838.4
|
10.6
|
256.3
|
1.8
|
|
Average
|
34.6
|
54.2%
|
76.8
|
1820.7
|
10.9
|
225.4
|
2.0
|
- Microarray targets prepared from donors A, C, and D were hybridized to the GeneChip Human Focus Array. Overall performance values determined with unscaled MAS 5.0 analysis of each array are listed. Bold font indicates metric values that fall below empirical quality control standards established in our laboratory. All arrays passed post-hybridization visual inspection. *Average values for Method 1 do not include sample A which was flagged as a low performance outlier.
