Figure 1
From: Activation of p53-regulated pro-apoptotic signaling pathways in PrP-mediated myopathy
Clustering of gene expression data. A. Measurements of relative gene expression for 6 time points (after 0, 4, 7, 14, 30, 60 days of Dox treatment) in Tg(HQK) mice (HQK) and PrP knockout mice (KO). Mice were treated with 6 g Dox/kg food, and three animals were taken at each time point as indicated. Total RNA was extracted from skeletal muscles (quadriceps) from the hind legs and subjected to microarray analysis, yielding expression profiles of genes with normalized expression ratios. Red and purple represent relative over-expression and under-expression, respectively, and the color intensity represents the magnitude of digression. B. Bioinformatic analysis (Ingenuity Pathways Analysis) to determine the top biological functions and associated p values of the selected genes is shown. The top four categories are listed for diseases and disorders, molecular and cellular functions, physiological system and development and function, and pathways associated with toxicity.