Figure 5

Total p53 protein level is up-regulated in the skeletal muscles of Tg(HQK) mice treated with doxycycline. Tg(HQK) mice were treated with 6 g Dox/kg food for 0–60 days as indicated, and three animals were taken at each time point. Skeletal muscles (quadriceps) from the hind legs were subjected to immunoblot analysis in three blots. Twenty micrograms of total proteins was loaded for each sample. Skeletal muscle (quadriceps) sample from an untreated wild type FVB mouse (WT) serves as the control to normalize data from the triplicate blots. A. A representative immunoblot probed with anti-p53 antibody followed by probing with an anti-actin antibody after stripping. B. Plot of the total p53 protein levels over increasing duration of Dox treatment. The p53 protein level for each sample was normalized against the actin level in each blot and expressed as the ratio against the normalized total p53 protein level in the untreated wild type FVB mouse on the same blot. The error bars denote standard errors calculated from the three blots. The bars with asterisk(s) indicate a statistically significant difference when compared to the 0 day Tg(HQK) samples. *p < 0.05; ** p < 0.01; *** p < 0.001.