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Table 1 Checklist of the 34 unique sequences obtained by SSH in post-vitellogenic ovaries of Blattella germanica

From: Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization

Sample

ESTs *

Length (bp)

ORF **

Best BLASTx

Species

E-value

GO (Molecular Function)

BG30001

19

2702

Yes

No homologya

-

-

-

BG30002

2

794

Yes

No homology

-

-

-

BG30003

52

1131

Yes

Cuticula protein-like a

Acyrthosiphon pisum

3.00E-07

Unknown

BG30004

2

347

Yes

Ribosomal protein L18A

Graphocephala atropunctata

9.00E-47

Structural constituent of ribosome

BG30005

2

367

No

No homology

-

-

-

BG30006

2

263

Yes

Ribosomal protein L24e

Nasonia vitripennis

1.00E-31

Structural constituent of ribosome

BG30007

2

171

Yes

No homology

-

-

-

BG30008

3

279

Yes

No homology

-

-

-

BG30009

91

1172

Yes

No homology

-

-

-

BG30010

2

193

Yes

No homology

-

-

-

BG30011

2

179

Yes

No homology

-

-

-

BG30012

5

195

No

EST LY_YIT_BG1527

Blattella germanica

2.00E -21

Unknown

BG30013

2

231

No

No homology

-

-

-

BG30014

19

117

No

No homology

-

-

-

BG30015

2

768

Yes

No homology

-

-

-

BG30017

28

1561

Yes

No homologya

-

-

-

BG30018

1

849

Yes

CG10407-PA-like

Tribolium castaneum

1.00E-23

Binding

BG30019

1

1616

Yes

Yellow-g a

Nasonia vitripennis

3.00E-29

Unknown

BG30020

Singlet

770

Yes

Baiser

Bombyx mori

4.00E-59

Unknown

BG30021

Singlet

523

Yes

Follicle cell protein 3c a

Nasonia vitripennis

7.00E-49

Unknown

BG30022

Singlet

317

Yes

Ribosomal protein L44e

Nasonia vitripennis

2.00E-40

Structural constituent of ribosome

BG30023

Singlet

191

Yes

Cabeza CG3606-PA-like

Tribolium castaneum

2.00E-06

Transcriptional activator activity

BG30024

Singlet

423

Yes

Ribosomal protein L36e

Nasonia vitripennis

2.00E-48

Structural constituent of ribosome

BG30025

Singlet

496

Yes

Ferritin 2

Acyrthosiphon pisum

5.00E-24

Ion binding

BG30026

Singlet

447

Yes

Troponin I

Aedes aegypti

3.00E-35

Protein binding

BG30027

Singlet

138

Yes

Cyclin B

Nasonia vitripennis

2.00E-06

Kinase regulator activity

BG30028

Singlet

136

Yes

Cathepsin-L a

Penaeus monodon

2.00E-08

Hydrolase activity

BG30029

Singlet

168

No

No homology

-

-

-

BG30031

Singlet

318

Yes

No homology

-

-

-

BG30032

Singlet

150

Yes

No homology

-

-

-

BG30033

Singlet

146

Yes

No homology

-

-

-

BG30036

Singlet

394

Yes

No homology

-

-

-

BG30037

Singlet

348

Yes

No homology

-

-

-

BG30038

Singlet

306

No

No homology

-

-

-

  1. The number of ESTs, the length of the sequence and the potential open reading frames (ORFs) are indicated. The 15 ESTs indicated in bold are homologous to known sequences. For these the best BLASTx match, the species, the E-value of the alignment, and the molecular function are indicated.
  2. a Selected for studying the expression in ovaries throught qRT-PCR
  3. * From subtractive library assemble against cDNA ovary library
  4. **Minimum length of 100 bp
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BMC Genomics

ISSN: 1471-2164

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