Figure 6
Neighbor joining guide phylogeny tree. Deduced amino acid sequences of 35 full-length serpins were aligned with 17 serpins from A. americanum [33], R. appendiculatus serpin (Ras) -1-to-4 [17], H. longicornis (Hl) serpin, I. ricinus (Irserp) serpin 1, 2, 4, 8 and Iris = immunosuppressive Ixodes serpin, I. scapularis (Is) serpin and B. microplus (Bm) serpin 1-to-6. Note that, except for Bmserpin-5, which, is annotated in GenBank, the other Bm serpins were obtained as ESTs from the TIGR database and translated in Mulenga et al., [33]. Cluster labels "A-to-E", represents branching from the outlier, the serpin superfamily archetype, α1-antitrypsin. Accession numbers of whole genome shotgun genomic DNA sequences corresponding to supercontig sequences used in the annotation of I. scapularis serpins (marked with an asterisk sign) are given in Table 3.