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Figure 8 | BMC Genomics

Figure 8

From: Ixodes scapularis tick ser ine p roteinase in hibitor (serpin) gene family; annotation and transcriptional analysis

Figure 8

Transcription profiles (8A and 8C) and normalized PCR band densities representing relative mRNA abundance (8B and 8D). cDNA synthesized from total RNA of salivary glands (SG) and midgut (MG) of unfed and ticks that were partially fed for 6 to 96 hrs was subjected to semi-quantitative RT-PCR using gene specific primers shown in tables 1 and 2. PCR band densities were determined using the ImageJ software http://rsb.info.nih.gov/ij. Determined densities were normalized using the following formula, Y = V + V(H-X)/X where Y = normalized mRNA density, V = observed Ispin PCR band density in individual tissues (MG and SG), H = highest actin PCR band density, X = tissue (MG and SG) actin band density.

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