Figure 1

Diagram of cDNA synthesis and 454 library preparation procedures. Three fragment types are produced by sonication: 5', internal, and 3'. Ligation with the partially double-stranded adaptors A and B produces, for each fragment type, certain adaptor configurations that will be amplified (above the '+'), and others that will be suppressed (below the '+') during the subsequent amplification. The procedure preferentially amplifies constructs that are appropriate for 454 sequencing (shown inside box).