Table 2 Validation of singleton sequences and the contig joining procedure by PCR amplification and Sanger sequencing.
From: Sequencing and de novo analysis of a coral larval transcriptome using 454 GSFlx
Singleton ID | PCR | Aligned region (bp) | % identity |
|---|---|---|---|
E60BDOM01CLWZX | + | 127 | 95% |
E60BDOM01ALGIK | + | Â | Â |
E60BDOM01BPLRZ | + | 154 | 94% |
E60BDOM02F1SCS | + | 148 | 99% |
E60BDOM01CV0LE | + | Â | Â |
E60BDOM01ETTGL | + | 157 | 99% |
E60BDOM01BOMBA | + | Â | Â |
E60BDOM01AM229 | Â | Â | Â |
E60BDOM01CMRH7 | Â | Â | Â |
E60BDOM01E1RA8 | + | 127 | 98% |
Scaffold ID | PCR | 5' match, bp (%identity) | 3' match, bp (%identity) |
EZ002257 | + | Â | 193 (98%) |
EZ000984 | + | 248 (99%) | 123 (99%) |
EZ001217 | + | 722 (96%) | 374 (98%) |
EZ001302 | + | 174 (97%) | 180 (99%) |
EZ001324 | + | 361 (97%) | 183 (99%) |
EZ002268 | + | 249 (99%) | 186 (99%) |
EZ001475 | + | Â | 323 (99%) |
EZ002219 | + | 715 (98%) | 218 (98%) |
EZ000750 | + | 768 (96%) | 212 (99%) |
EZ001662 | + | 133 (99%) | 55 (98%) |