Table 3 Validation of the differences in gene expression determined by the array analysis using real-time PCR of gene expression in a test set of tumors.
Gene | Tumor set 1 (Microarray analysis, arbitrary units) | Tumor set 2 (test set) (Real-Time PCR analysis, 2-Δct × 1,000) | ||||
|---|---|---|---|---|---|---|
|  | BLG/STAT5Δ750 tumors | BLG/STAT5ca tumors | Fold change (BLG/STAT5Δ750/BLG/STAT5ca) | BLG/STAT5Δ750 tumors | BLG/STAT5ca tumors | Fold change (BLG/STAT5Δ750/BLG/STAT5ca) |
Edg3 | 1.59 ± 0.21 | 0.43 ± 0.15 | 3.64 | 0.10 ± 0.02 | 0.04 ± 0.01 | 2.08 |
Mapk8 | 1.30 ± 0.19 | 0.51 ± 0.12 | 2.54 | 0.64 ± 0.189 | 0.43 ± .05 | 1.49 |
Wnt8a | 1.41 ± 0.31 | 0.44 ± 0.14 | 3.15 | 1.41 ± 0.380 | 0.37 ± 0.21 | 3.75 |
Ptn | 1.69 ± 0.48 | 0.46 ± 0.25 | 3.68 | 1.01 ± 0.219 | 0.48 ± 0.07 | 2.09 |
Foxk1 | 3.49 ± 0.97 | 0.86 ± 0.19 | 4.04 | 0.35 ± 0.118 | 0.25 ± 0.02 | 1.42 |
Tyrp1 | 1.37 ± 0.21 | 0.41 ± 0.17 | 3.33 | 2.22 ± 1.159 | 0.47 ± 0.18 | 4.71 |