Figure 1

RT-PCR analysis of the ZNF191 gene expression in HEK293 cells transfected with pcDNA3-ZNF191 or pcDNA3 and untreated cells. (A) Semiquantitative RT-PCR analysis of the ZNF191 gene expression in the transfected cells and untreated cells. Total cellular RNA was isolated from pcDNA3-ZNF191 or pcDNA3 transfectants and untreated cells, and, after reverse transcription, PCR was performed with ZNF191- and GAPDH- specific primers. The up-regulation of the 481-bp ZNF191-specific band was detected in cells transfected with pcDNA3-ZNF191 plasmid. (B) Quantitative RT-PCR analysis of the relative ZNF191/GAPDH expression in the transfected cells and untreated cells.