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Figure 3 | BMC Genomics

Figure 3

From: Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling

Figure 3

Statistical analysis of expression level measurements for assessing reproducibility and comparability of amplification chemistries. A, we calculated the Pearson's correlation coefficients between technical replicates (same chemistry, same amount of input RNA, but different laboratories). The correlation values were then averaged for each chemistry (grey bars). We also calculated correlation coefficients between results obtained from 250 pg and from 500 pg of RNA input (same chemistry, but different amount of RNA input) and averaged them for each chemistry in order to evaluate the robustness across quantitative variability of RNA input (black bars). Most Epicentre amplifications did not yield sufficient aRNA to carry out hybridisations, and Pearson's correlation coefficients could therefore not be calculated (*). B, Graphic representation of Pearson's correlation coefficients calculated for each pairwise comparison of all assays.

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