Figure 1

Principles of the two target preparation methods used to assay both sense- and antisense-strand transcription. A 5' (head-to-head) overlapping sense-antisense transcript pair is used as an example. The standard Affymetrix 3' in vitro transcription (3'IVT) assay was used to detect sense-strand transcription, while a modified Affymetrix Whole Transcript (WT) assay was used to detect antisense-strand transcription.