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Table 2 Validation of selected sense-antisense transcript pairs with strand-specific reverse transcription PCR (RT-PCR).

From: Large-scale analysis of antisense transcription in wheat using the Affymetrix GeneChip Wheat Genome Array

Probe set ID

Putative function1

RT-PCR result2

  

Germinated seed

Shoot

Flag leaf

Spike pre-anthesis

Spike post-anthesis

  

S

A

S

A

S

A

S

A

S

A

Ta.136.1

Thioredoxin

X

 

X

X

X

X

X

X

X

X

Ta.2752.2

RUBISCO small subunit

X

 

X

X

X

X

X

X

X

X

Ta.27660.1

RUBISCO activase

  

X

 

X

 

X

 

X

 

Ta.5227.1

Carbonic anhydrase

X

 

X

X

X

X

X

X

X

X

TaAffx.128414.1

RUBISCO large subunit

X

X

X

X

X

X

X

X

X

X

TaAffx.128418.24

Putative senescence-associated protein

X

X

X

X

X

X

X

X

X

X

TaAffx.128757.1

Photosystem Q(B) protein

X

X

X

X

X

X

X

X

X

X

TaAffx.129824.1

Translation initiation factor

X

X

X

X

X

X

X

X

X

X

TaAffx.4530.1

ATP synthase alpha chain

X

 

X

X

X

X

X

X

X

X

TaAffx.4544.2

Cytochrome c biogenesis protein ccsA

X

 

X

X

X

X

X

X

X

X

  1. 1Functional categories were based on the Munich Information Center for Protein Sequences classifications and putative function shows the best significant BLASTX database hit from HarvEST.
  2. 2RT-PCR result shows detection of transcription (X) for the sense (S) strand and antisense (A) strand in each tissue.
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BMC Genomics

ISSN: 1471-2164

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