Table 1 Primer sequence, annealing temperature [AT] (degrees Celsius – °C) and fragment length [FL] (base pairs – bp) for genes amplified using Q- PCR.
Gene Name | Forward Primer | Reverse Primer | AT (°C) | FL (bp) |
|---|---|---|---|---|
Galectin 3 | CTGGATACCCAGGTGCCTAT | AGTACGGTGCAGTTGGTCCT | 55 | 180 |
IFNAR1 | CTTTGGGATCCTCGTTCTGA | GCAACACAGGAGGCTTTCAT | 55 | 201 |
Mannose-6-P-R | AAGCTAATGAGAATGAAACGGAGTG | CATCTGGGATGGTTAACACTTCATC | 60 | 180 |
MCG24 | TCTGTACAAATTCTGCAGGTCGAAA | AGAGATTCACATTACCTCTTGCCAA | 55 | 222 |
TCRγ | CACATGACACACAAGGCAGCA | TAGCACAGAGAGCAGGAGAGCTTAT | 55 | 225 |
Granzyme A | TGGGTGTTAACAGCTGCTCATTGC | GGGGAATGACTTTCACAGCGCTA | 52 | 213 |
IgY R | AGGAGAACCCGGCCACAC | ATCTGACCACTGCCAGCCA | 60 | 180 |