Gene expression profiling within the spleen of Clostridium perfringens- challenged Broilers fed antibiotic-medicated and non-medicated diets

Table 3 Validation of microarray results with quantitative PCR assays.

Gene Name	Post-/pre- challenge	Direction of Fold Change in Expression Data		Validation Success
		Medicated	Non-medicated
	D1/D0	+	+
Granzyme A	D2/D0	+	+	6/6 (100%)
	D4/D0	+	+
	D1/D0	+	+
IFNAR1	D2/D0	+	+	5/6 (83%)
	D4/D0	+	*
	D1/D0	+	N/A
Galectin 3	D2/D0	+	+	3/5 (60%)
	D4/D0	*	*
	D1/D0	+	N/A
Mannose 6-P-R	D2/D0	+	+	4/5 (80%)
	D4/D0	+	*
	D1/D0	+	+
TCRγ	D2/D0	+	N/A	5/5 (100%)
	D4/D0	+	+
	D1/D0	+	+
MCG24	D2/D0	N/A	N/A	2/3 (66.7%)
	D4/D0	N/A	*
	D1/D0	+	+
IgY R	D2/D0	+	+	5/6 (83%)
	D4/D0	*	+

N/A: No significant difference between time points was determined from microarray analysis and no quantitative PCR validation was therefore performed.
*Direction of fold change was conflicting between microarray and quantitative PCR results.

ISSN: 1471-2164