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Table 1 Validation of the selected microarray data by the qRT-PCR.

From: Molecular signature of cell cycle exit induced in human T lymphoblasts by IL-2 withdrawal

Rank Gene symbol Description Probe set Signal Log Ratio (microarray analysis) Micorarray
Fold-change
(after/before
IL2 withdrawal)
Parametric
p-value
(non-paired
comparison)
qRT-PCR Fold change qRT-PCR p-value (non-paired)
     j 43 6 line 5 S9     
genes with P < 0.0001 in the microarray gene expression analysis
2 CISH cytokine inducible SH2-containing protein 223377_x_at -5.01 -4.936 -5.435 -4.649 -4.519 0.03 <0.0001 0.08 <0.0001
7 DUSP6 dual specificity phosphatase 6 208893_s_at -6.236 -5.771 -5.75 -4.873 -5.028 0.02 <0.0001 0.04 <0.0001
11 C3orf59 chromosome 3 open reading frame 59 227599_at -3.035 -2.559 -2.647 -2.401 -2.29 0.17 <0.0001 0.32 0.0082
18 ASAH1 N-acylsphingosine amidohydrolase (acid ceramidase) 1 213702_x_at 0.726 0.671 0.612 0.569 0.787 1.59 <0.0001 2.08 0.0069
genes with P < 0.001 in the microarray gene expression analysis
33 SOCS2 suppressor of cytokine signaling 2 203373_at -4.178 -4.954 -5.332 -3.528 -4.438 0.04 0.0001 0.12 0.0058
45 OSM oncostatin M 214637_at -4.201 -4.671 -3.352 -4.564 -5.351 0.04 0.0002 0.04 0.0011
66 IL8 interleukin 8 211506_s_at -3.537 -2.395 -3.14 -4.011 -3.575 0.10 0.0003 0.19 0.2333
92 HBEGF heparin-binding EGF-like growth factor 203821_at -5.121 -3.605 -4.72 -3.679 -3.178 0.06 0.0004 0.08 0.0001
genes with P < 0.01 in the microarray gene expression analysis
221 MT1F metallothionein 1F 217165_x_at 2.408 1.794 1.091 2.344 2.002 3.80 0.0013 7.05 0.0004
348 MT1X metallothionein X 208581_x_at 2.066 1.764 0.997 2.656 2.005 3.73 0.0021 4.76 0.0004
514 CLK4 CDC-like kinase 4 210346_s_at 1.399 1.014 1.729 1.188 0.576 2.27 0.0036 2.41 0.0046
600 IL7R interleukin 7 receptor 205798_at 1.923 0.919 1.965 2.962 1.721 3.73 0.0043 3.19 0.2734