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Table 1 Validation of the selected microarray data by the qRT-PCR.

From: Molecular signature of cell cycle exit induced in human T lymphoblasts by IL-2 withdrawal

Rank

Gene symbol

Description

Probe set

Signal Log Ratio (microarray analysis)

Micorarray

Fold-change

(after/before

IL2 withdrawal)

Parametric

p-value

(non-paired

comparison)

qRT-PCR Fold change

qRT-PCR p-value (non-paired)

    

j

43

6

line 5

S9

    

genes with P < 0.0001 in the microarray gene expression analysis

2

CISH

cytokine inducible SH2-containing protein

223377_x_at

-5.01

-4.936

-5.435

-4.649

-4.519

0.03

<0.0001

0.08

<0.0001

7

DUSP6

dual specificity phosphatase 6

208893_s_at

-6.236

-5.771

-5.75

-4.873

-5.028

0.02

<0.0001

0.04

<0.0001

11

C3orf59

chromosome 3 open reading frame 59

227599_at

-3.035

-2.559

-2.647

-2.401

-2.29

0.17

<0.0001

0.32

0.0082

18

ASAH1

N-acylsphingosine amidohydrolase (acid ceramidase) 1

213702_x_at

0.726

0.671

0.612

0.569

0.787

1.59

<0.0001

2.08

0.0069

genes with P < 0.001 in the microarray gene expression analysis

33

SOCS2

suppressor of cytokine signaling 2

203373_at

-4.178

-4.954

-5.332

-3.528

-4.438

0.04

0.0001

0.12

0.0058

45

OSM

oncostatin M

214637_at

-4.201

-4.671

-3.352

-4.564

-5.351

0.04

0.0002

0.04

0.0011

66

IL8

interleukin 8

211506_s_at

-3.537

-2.395

-3.14

-4.011

-3.575

0.10

0.0003

0.19

0.2333

92

HBEGF

heparin-binding EGF-like growth factor

203821_at

-5.121

-3.605

-4.72

-3.679

-3.178

0.06

0.0004

0.08

0.0001

genes with P < 0.01 in the microarray gene expression analysis

221

MT1F

metallothionein 1F

217165_x_at

2.408

1.794

1.091

2.344

2.002

3.80

0.0013

7.05

0.0004

348

MT1X

metallothionein X

208581_x_at

2.066

1.764

0.997

2.656

2.005

3.73

0.0021

4.76

0.0004

514

CLK4

CDC-like kinase 4

210346_s_at

1.399

1.014

1.729

1.188

0.576

2.27

0.0036

2.41

0.0046

600

IL7R

interleukin 7 receptor

205798_at

1.923

0.919

1.965

2.962

1.721

3.73

0.0043

3.19

0.2734