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Table 5 Percentages of apoptotic and necrotic cells in cultures of IL-2-dependent primary (j, 43) and immortalised (line 5, S9) T lymphoblasts (0 h) and at different time points after IL-2 withdrawal (2 h, 8 h, 24 h), analysed by flow cytometry following Annexin-V staining.

From: Molecular signature of cell cycle exit induced in human T lymphoblasts by IL-2 withdrawal

Cell lines 0 h1 2 h 8 h 24 h
  A2 N3 A N A N A N
j 0.24 2.1 0.9 1.1 0.4 2.2 0.5 2.0
43 0.5 3.0 0.7 2.0 0.4 2.2 0.7 3.0
line 5 2.3 4.0 3.8 2.3 4.6 2.0 8.1 3.8
S9 5.4 1.3 6.7 1.3 8.4 1.5 6.9 1.1
  1. 1hours after IL-2 withdrawal
  2. 2A – apoptosis
  3. 3N – necrosis
  4. 4percentage; mean of two replicates