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Table 5 Percentages of apoptotic and necrotic cells in cultures of IL-2-dependent primary (j, 43) and immortalised (line 5, S9) T lymphoblasts (0 h) and at different time points after IL-2 withdrawal (2 h, 8 h, 24 h), analysed by flow cytometry following Annexin-V staining.

From: Molecular signature of cell cycle exit induced in human T lymphoblasts by IL-2 withdrawal

Cell lines

0 h1

2 h

8 h

24 h

 

A2

N3

A

N

A

N

A

N

j

0.24

2.1

0.9

1.1

0.4

2.2

0.5

2.0

43

0.5

3.0

0.7

2.0

0.4

2.2

0.7

3.0

line 5

2.3

4.0

3.8

2.3

4.6

2.0

8.1

3.8

S9

5.4

1.3

6.7

1.3

8.4

1.5

6.9

1.1

  1. 1hours after IL-2 withdrawal
  2. 2A – apoptosis
  3. 3N – necrosis
  4. 4percentage; mean of two replicates