Table 2 Primer pairs and corresponding annealing temperatures used for successful amplification of mitochondrial genome fragments from Sipunculus nudus
Primer names | Primer sequence (5'-3') | Annealing temperature | Approxim. size of PCR product |
|---|---|---|---|
Sn-cox1-f | CTCCCACTTAGCACCCTC | 48°C | 400 bp |
Sn-cox2-r | TAAGAGAATAATGGCGGG | Â | Â |
Sn-cox2-f | CCAACCACTCTTTTATGCC | 50°C | 700 bp |
Sn-cox3-r | CCAGGATTAGGGCGGT | Â | Â |
Sn-cox3-f | TTTTCCTATACCTCTGCATC | 48°C | 700 bp |
Sn-cob-r | TTGAATGACAAGGCAGAGA | Â | Â |
Sn-cob-f | CTCCTCGCCCCCAA | 48°C | 2500 bp |
Sn-16S-r | GATTTATTGAAGAGTGGTTAGTGA | Â | Â |
Sn-16S-f | TCATACCCCGCACTCC | 48°C | 600 bp |
Sn-nd3-r | CAAACCCGCACTCAAAC | Â | Â |
Sn-nd3-f | GGTAAGTGAACGGGGAAC | 50°C | 2500 bp |
Sn-nd1-r | AAAAGGTTGGGGGAGG | Â | Â |
Sn-nd1-f | CGCTATCACCTCCACCTT | 50°C | 4000 bp |
Sn-cox1-r | ATTCGGCACGGATAAGA | Â | Â |