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Table 2 Validation of microarray results by quantitative RT-PCR.

From: Maternal diabetes alters transcriptional programs in the developing embryo

 

Microarray

qRT-PCR

Gene Symbol

Fold change

t-test (p-value)

Fold change

t-test (p-value)

Adam10*

-2.37

0.0024

-1.56

0.035

Api5*

-2.30

0.0004

-1.33

0.007

Atrx

-2.15

0.0016

-1.33

0.046

Baz1b*

-2.38

0.0012

-1.58

0.017

Cxadr*

-2.00

0.0034

-1.38

0.0002

Dcx*

-3.05

0.0001

-2.20

<0.0001

Efnb2*

-2.04

0.0437

-1.72

0.010

Hif1a

-2.52

0.0041

-1.49

0.011

Il6st

-2.28

0.0198

-1.77

0.039

Mt2

3.45

0.0053

2.16

0.027

Mtap2

-3.02

0.0000

-1.98

0.0002

Pcdh18*

-5.77

0.0017

-1.35

<0.0001

Pdgfra1

-2.01

0.0100

-1.42

0.018

Tgfβ r1

-3.26

0.0002

-1.89

0.010

Twsg1

-4.91

0.0002

-1.89

0.005

Vcl

-2.37

0.0020

-2.03

0.002

Hmga1

2.13

0.0060

-2.14

<0.0001

Lin28*

2.00

0.0182

-1.30

0.032

Ogt

2.52

0.0310

-1.51

0.019

Slc2a1

2.15

0.0252

-1.68

0.012

Phip

-2.76

0.0006

1.49

0.011

Rod1*

-2.32

0.0004

1.20

0.041

Sema3a

-2.05

0.0003

1.74

0.024

Creb1

-3.33

0.0007

1.05

ns

Cited4*

2.11

0.0026

-1.10

ns

Tfrc*

-2.20

0.0023

1.11

ns

  1. For each gene, the amplification rate was calculated from the linear range of the reaction, and diabetes-exposed embryos (n = 9 from 4 litters) were compared to 4 pools (4 litters) of mRNA from control embryos. In reactions for genes marked with *, the control group consisted of mRNA from individual embryos (n = 6 from 4 litters). All embryos were isolated at E10.5 and were morphologically normal. Negative values for "fold-change" indicate reduction of expression.
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BMC Genomics

ISSN: 1471-2164

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