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Table 2 Validation of microarray results by quantitative RT-PCR.

From: Maternal diabetes alters transcriptional programs in the developing embryo

  Microarray qRT-PCR
Gene Symbol Fold change t-test (p-value) Fold change t-test (p-value)
Adam10* -2.37 0.0024 -1.56 0.035
Api5* -2.30 0.0004 -1.33 0.007
Atrx -2.15 0.0016 -1.33 0.046
Baz1b* -2.38 0.0012 -1.58 0.017
Cxadr* -2.00 0.0034 -1.38 0.0002
Dcx* -3.05 0.0001 -2.20 <0.0001
Efnb2* -2.04 0.0437 -1.72 0.010
Hif1a -2.52 0.0041 -1.49 0.011
Il6st -2.28 0.0198 -1.77 0.039
Mt2 3.45 0.0053 2.16 0.027
Mtap2 -3.02 0.0000 -1.98 0.0002
Pcdh18* -5.77 0.0017 -1.35 <0.0001
Pdgfra1 -2.01 0.0100 -1.42 0.018
Tgfβ r1 -3.26 0.0002 -1.89 0.010
Twsg1 -4.91 0.0002 -1.89 0.005
Vcl -2.37 0.0020 -2.03 0.002
Hmga1 2.13 0.0060 -2.14 <0.0001
Lin28* 2.00 0.0182 -1.30 0.032
Ogt 2.52 0.0310 -1.51 0.019
Slc2a1 2.15 0.0252 -1.68 0.012
Phip -2.76 0.0006 1.49 0.011
Rod1* -2.32 0.0004 1.20 0.041
Sema3a -2.05 0.0003 1.74 0.024
Creb1 -3.33 0.0007 1.05 ns
Cited4* 2.11 0.0026 -1.10 ns
Tfrc* -2.20 0.0023 1.11 ns
  1. For each gene, the amplification rate was calculated from the linear range of the reaction, and diabetes-exposed embryos (n = 9 from 4 litters) were compared to 4 pools (4 litters) of mRNA from control embryos. In reactions for genes marked with *, the control group consisted of mRNA from individual embryos (n = 6 from 4 litters). All embryos were isolated at E10.5 and were morphologically normal. Negative values for "fold-change" indicate reduction of expression.