A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms

Table 2 Numbers of positive clones detected with Illumina oligonucleotide primers in the 1× genome equivalent of the Eco RI Ae. tauschii BAC library and the positive clones detected in contig in the remaining BAC and BiBAC libraries.

Locus	Map position (cM)	Contig	All positive clones in the contig	No. positive clones in 1× genome equivalent
BF428792	66.5	ctg11447	HD091B17, HB035D2, HB048A19, HD131L18, RI135A13, HI004M3	0
BE471274	89.2	ctg487	BB037M12, HB007I22, HB057P16, HB085A14, HI006N2, HD032D6, HI031J11	0
BE590745	101.2	ctg11005	HB132A7, RI076L24, HI039I15	0
BE591939	102.3	ctg6341	HD086O22, RI011N13, RI050D20*, RI053E15, HI059D10, HD120B16, HI105L1	1
BF201348	102.4	ctg9913	BB005K5, BB003G23, BB051C23, BB106M18, RI074C16, HI056I10	1
BM137697	121.4	ctg5641	BB054M2, RI041O13, RI140B8	0
BQ168191	93.0	ctg4237	HD145O11, HD088L23, RI089E13, HI030D11	0
BE471132	0.00	ctg6261	HD080D15, HD023E5, RI005I8, HD124N6, HI042G4, HI104G24	1
BE445628	85.9	ctg3619	RI130I10, HI112C21	0
CD452951	103.1	ctg4985	HD076J10, HD004P4, HD059D21, RI144L6	0
BE403177	109.0	ctg5209	BB014B15, BB012C19, HD087J6, RI048C1, RI090E18, HI014F23, HI146N21	2
BE399200	109.8	ctg5429	RI048D18, BB028N11, RI114F3, RI127A20	2
BE406351	113.6	ctg3299	HD085I19, HD133F6, RI064D18	0
BE444599	143.4	ctg19	HB012E19, HB084C20, RI074P11, HD028J11	1
BE445431	151.5	**	RI119I14, RI101I14, RI125I14	3

* Clones in bold were detected with Illumina and PCR in the 1× genome equivalent of the Eco RI library. ** Clones were detected but were not fingerprinted.

ISSN: 1471-2164