Figure 4

Validation of microarray results for genes similarly affected by strain irrespective of acute cardiac rejection. Two inflammatory response genes, Ccl9 and Itgal, were selected from the peripheral blood mononuclear cell (PBMC) overlap category shown in Figure 3B. Microarray and qRT-PCR results are from the same animals. (A) Strain effect in PBMC shown as the fold change in gene expression comparing untransplanted BN and DA rats. (B) Strain plus rejection effects in PBMC shown as the fold change in gene expression comparing allogeneic transplant recipients (BN) and isogeneic transplant recipients (DA). Ccl9 expression was higher and Itgal was lower in BN compared to DA animals irrespective of transplant status across both platforms.