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Table 3 Number of positive clones on Heliothis virescens library filters.

From: Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species

Library

HVR 7.3X

HVB 9.0X

Expected 16.3X

 

Probe

Function

Hits per library

Hits per library

Total Hits

Probe source

K390

cap binding protein

7

14.8

22

R. Palli

K386°

ribosomal protein S4

10

1.1

11

R. Palli

L086°

TIA-1

5

5.4

10

R. Palli

APN120°

Midgut receptor

8

3

11

S. Gill

Cadherin-650

Bt receptor

5.8

4.4

10

L. Gahan

Cadherin-1 kb

Bt receptor

3

--

3

S. Gill

HR 16

Olfactory receptor

--

17

17

F. Gould

PTTH

peptide hormone

--

18

18

PCR/GenBank

 

Ave. no. hits per probe

7.6

9.1

16.7

 
  1. Probes were derived from bacterial plasmids except for PTTH. Decimals in hits reflect extrapolation for filters that were not screened.