Figure 1

Workflow of cis-element discovery in rice and sorghum. Two complementary approaches have been employed for motif discovery. Starting from orthologous gene pairs between rice and sorghum, orthologous upstream sequences have been isolated. These sequences were used to identify motifs with a high conservation rate between syntenic pairs compared to their single genome frequencies (network-level conservation approach). For PhyloCon, orthologous pairs that were supported by co-expression were combined to orthologous groups and were subjected to a PhyloCon analysis. Motifs were consecutively filtered for repeats and merged by clustering. Statistical significance has been re-evaluated for each of the clustered motifs. For further details, see text and Methods.