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Table 4 Q-PCR primer pairs used to amplify selected barley and wheat transcripts.

From: Comparative transcriptomics in the Triticeae

Probeset

Forward primer

Reverse primer

Product size (bp)

Barley1

   

Contig8230

TACATGCTCTTGTTTGGTGCTACTG

AAGGTAAGTAGGCAGCAGTGAAGGT

204

Contig6943

GGGGAAATCCCAGGTCGTCGAT

GGCTTGCTGCTAGGGTTTTCAG

266

Contig7925

CGAACCGTAGAATGTGTAAGGG

GGGAGGAAAGATACACGCTT

114

Contig3031

TTACTATGCTGGATATGGACAAGGG

TCTCATCTCATGTCTGGAAGACCC

190

Contig4668

CCCCCCACAAGTACCTGAAGA

CGTTGGCTTGCTTAGCTCTTCC

286

Contig7671

CTAAGCGACCTTGCATCTTTTGAC

AACGCTAGTGCTACTGGCAGGA

213

Contig20269

GAAGGCTCAGAAAGTTGCTGCTAT

GCAAAATCATTCACTGCTTCCAGAG

224

Contig5740

GAGGCTGTTCAGCAACTGGACTG

CAAGGATCCCAGCCACATACTG

227

Contig15148

GATCTCTTCGTGGTGGATCACATAC

GCTTGATGTCCTATGCTTTCCAA

221

Contig11660

ACCTCATCAACCTCTGCGGC

TTCCAGAGAACGGAGGCAGG

210

Contig14399

AGAAAGAGAGATTTTGAAGCTTGGC

AATCCATCGCCATGCCAACT

213

Contig15147

GGCGGGGCACTTTTGAGGACAT

CGAGCCTGCGACGGGTTATT

182

Contig2400

AAGCATGCCGCCATCCCGTT

CCCAACCTGACAACTCCACCTAGA

244

Wheat

   

Ta.3039.1

ACGTCCATAACGATGGTCTTCATTG

GTAGTGGCCTCAGCATCACCATTGC

170

Ta.13729.1

TTTTCTACATGCTCTTGTTTGGTGC

AAAAGATCAACCCATGTGCTGCTCC

265

Ta.27013.1

CGAAGCGTGTATCTTTCCTC

CAGACACAAACGAAAATGAC

183

Ta.7602.1

AGCCCCCCACAAGTACCTGATGATG

GTCGTCATCCTCGTCACCATCTTCC

201

Ta.27369.1

TTACTATGCTGGATATGGACAAGGC

TGCTACAACATTAGCCTTGACAGTG

230

Ta.9536.1

GCCCTAAACGACCTTGCATCTTTTG

AAACTGAAGCACTAACCTACGACGC

236

Ta.968.1

ATGTGCTGCGTCGTCAGATACATAG

TACCCTCCTCGACTTCCTTGTGATC

204

Ta.4425.1

GATGCCATCAGATCCTCCAATT

GCCACTCCGTTGTGTCATAATATGG

235

Ta.27038.1

CGAAGCGTGTATCTTTCCTC

CAGACACAAACGAAAATGAC

151

Ta.7256.1

CATCTCATGGTACCTGACTGTCGA

GCAACAGACTGCCACCAGCA

264

TaAffx.46790.1

TCATGTCAGTTTATTGCAAGG

CAGTGACACTATAACAATACAGTTCT

240

TaAffx.128707.1

GAAAAGGTTGTAGTTCAGAAGG

TTGCTCTGGACTACTGTCTTC

259

  1. Primer sequence details used in the Q-PCR verification of selected barley and wheat expression profiles and the size of the corresponding RNA products.